Evaluation of the revised serum calcium procedure used with the Du Pont automatic clinical analyzer.
نویسندگان
چکیده
We evaluated a revised serum calcium method (o-cresolphthalein complexone) used with the Du Pont aca. Our results correlated well with those obtained by atomic absorption spectrometry. Within-run and day-to-day coefficients of variation were 0.5 and 1.5%, respectively. Color produced and calcium concentration are linearly related and the relation is not affected by hemolysis, lipemia, or icterus.
منابع مشابه
Hemoglobin interference with Du Pont Automatic Clinical Analyzer procedure for calcium.
Hemoglobin in concentrations of approximately 2.5 g/liter or greater significantly increases values for apparent calcium in serum as determined with the Du Pont aca, an interference attributable to the 575-nm absorption band of oxyhemoglobin being uncompensated by the 577--600 nm differential absorption reading of the calcium o-cresolphthalein complexone complex.
متن کاملSource of error in cerebrospinal fluid protein determination with the Du Pont Automatic Clinical Analyzer.
To the Editor: The most widely used method for measurement of cerebrospinal fluid (CSF) total protein is turbidimetry. We use the Du Pont Automatic Clinical Analyzer (aca; Du Pont Instruments, Wilmington, Del. 19898) for this measurement. In this method, CSF proteins are precipitated by trichloroacetic acid and the resulting turbidity is measured spectrophotometrically (1). Values are falsely l...
متن کاملAn evaluation of the Du Pont aca IV: does it meet medical needs?
A new bench-top analyzer from Du Pont, the "aca IV," was evaluated. To do so, we performed 10 different tests during an eight-week test period. The instrument is very precise (CVs less than 2%), results agree well with those obtained with the older acas (II and III), linearity is good in the clinically important ranges, and there is very little drift or carryover. From a medical-needs viewpoint...
متن کاملEvaluation of the enzymatic ammonia method for urine on the Du Pont automatic clinical analyzer.
The enzymatic method for ammonia with the Du Pont Automatic Clinical Analyzer was tested in our laboratory. The method is very suitable for urine samples although the method was released by Du Pont only for plasma samples. The results correlated well with those obtained by a method based on the Berthelot indophenol reaction and by a method based on the use of an ammonium electrode, which has an...
متن کاملDevelopment and performance of a fully automated method for assay of C-reactive protein in the aca discrete clinical analyzer.
A quantitative immunoassay for C-reactive protein (CRP) has been developed for use in the Du Pont aca discrete clinical analyzer. Particle-enhanced turbidimetric immunoassay (PE-TIA) technology is used. The method has a CV of less than 10% in the range 2 to 120 mg/L. Neither hemolyzed samples (Hb less than 5 g/L), icteric samples (total bilirubin less than 300 mg/L), lipemic samples (triglyceri...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 21 7 شماره
صفحات -
تاریخ انتشار 1975